RESUMO
Our previous study has showed that a novel gene is differentially expressed in the liver of cyadox-fed piglets, but its sequence and function are unknown. Here, rapid amplification of cDNA ends (RACE) and bioinformatics analysis showed that the novel gene is 953 bp without protein-coding ability and locates in chromosome 11. Hence, we identified the novel gene as long non-coding RNA (lncRNA) and named it cyadox-related novel gene (CRNG). Fluorescence in situ hybridization (FISH) showed that CRNG mainly distributes in cytoplasm. Moreover, microarray assay in combination with CRNG interference and overexpression showed that the differential genes such as ANPEP, KITLG, STAT5A, FOXP3, miR-451, IL-2, IL-10, IL-6, and TNF-α are mainly involved in viral and pathogens infection and the immune-inflammatory responses in PK-15 cells. This work reveals that CRNG might play a role in preventing the host from being infected by pathogens and viruses and exerting immune regulatory effects in the cytoplasm, which may be involved in prophylaxis of cyadox in piglets.
RESUMO
OBJECTIVE: To investigate the knowledge, attitude of the AIDS prevention and occupational safety in dentist in Kunming and west part of Yunnan and evaluate the effect of education and intervention. METHOD: 165 dentists in Yunnan were tested by KABP questionnaire before education. All of the 165 dentists participated a course on AIDS prevention. After that course the same questionnaire was answered by participants. RESULT: The correct rates obviously increased. The understanding rates of the oral prevention measure were obviously improved. CONCLUSION: The knowledge of AIDS prevention is very low in dentist in Kunming and west part of Yunnan. The education intervention can effectively improve the understanding level of the AIDS prevention in dentist.
